Patient-specific neuro–immune mouse avatar model

The PSniM-Avatar model aims to create a next-generation patient-specific model of glioblastoma that captures the complex interactions between tumor cells, human neural tissue, immune components, and the vascularized brain microenvironment. This platform integrates patient-derived iPSCs, matched cerebral organoids, microglia, and glioma stem cells to generate personalized GLICO and immune-GLICO models, which are then adapted for in vivo engraftment and longitudinal analysis.

We have established foundational components of the system, including patient-specific organoid and iGLICO generation, successful engraftment and vascularization within the mouse brain (as shown in Wang et al., 2024 for astrocytic cortical organoids), and dual-luminescence imaging combined with MRI to track both tumor and organoid compartments over time.

By enabling direct interrogation of tumor–host communication in a more complete neuro–immune–vascular context, the PSniM-Avatar model is designed to uncover actionable vulnerability hubs that may drive glioblastoma adaptation, resistance, and therapeutic response.